CRISPR-Nanoparticle delivers Cas9 RNP and donor DNA into a wide variety of cell types and is able to correct the DNA mutations.
gRNA and donorDNA engineering
We have identified several gRNA and donor DNA modifications that enhance cell delivery of CRISPR/Cas9 therapeutics, and also enable enrichment of gene edited cells. This approach is based on terminal modifications of the gRNA and donor DNA, and has numerous applications DNA for genome engineering.
For example, we have synthesized a gRNA and donor DNA conjugate and observed that this conjugate increases the HDR efficiency of cells transfected with the CRISPR/Cas9 system.